Bay Area Lyme Tick Testing Team with Dr Nate Nieto (right)

Bay Area Lyme Foundation’s Free Tick Testing Program has ended and is no longer accepting ticks for testing. We thank all of the Citizen Scientists who participated. It was a rousing success—we received and tested over 21,000 ticks representing every state.

Research Project Overview

In February 2016, Bay Area Lyme Foundation became the first to offer free tick testing for residents of the United States. Testing was available through a partnership with Nate Nieto’s Lab at Northern Arizona University. With a citizen science focus, the crowd-sourced data was a vehicle for gaining a greater understanding of the geography of tick-borne diseases in the U.S.

Bay Area Lyme Tick Testing programDuring 2016 through 2018, ticks sent to the initiative were tested free of charge for four of the most common bacterial infections: Borrelia burgdorferi, which causes Lyme disease; Borrelia miyamotoi, which causes tick-borne relapsing fever; Anaplasma phagocytophilum, which causes human granulocytic anaplasmosis, and the protozoan pathogen, Babesia microti. These data were categorized, mapped, and recorded, as well as provided to the submitter.

The research project tested a massive sample of more than 21,000 ticks, collected from 49 U.S. states (all states except Alaska) and Puerto Rico. The program received a six-fold increase in tick submissions over initial estimates, representing unprecedented national coordination of a ‘citizen science’ effort and diagnostic investigation.

Research Findings

This citizen science program found ticks capable of carrying Lyme and other tick-borne diseases in 83 counties (in 24 states) where these ticks had not been previously recorded. The 24 states that contain counties with newly documented Ixodes scapularis (deer tick) or Ixodes pacificus (Western black-legged tick) populations are:  Alabama, Arizona, Georgia, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Michigan, Minnesota, Montana, Missouri, Nevada, North Carolina, Ohio, Oregon, South Carolina, Tennessee, Texas, Utah, Virginia, Washington, and Wisconsin. The map below illustrates this important finding.


Other interesting research findings include:

  • Anaplasma, Babesia, and Borrelia pathogens were found in all three of the most commonly encountered ticks collected—Ixodes, Amblyomma and Dermacentor species.
  • All life stages of these three tick species, including some larvae, were found to be infected with both Borrelia burgdorferi and Borrelia miyamotoi.
  • Several Amblyomma americanum, which is commonly known as the Lone Star tick and capable of carrying bacteria that cause disease in humans, were found in Northern California, the first known report of this tick in the state.

New data from the same study published in PLOS ONE (1/5/21) predicts and examines large-scale tick distributions on the Pacific Coast, highlighting areas of high risk of Lyme disease. This publication underscores the benefits of citizen science pointing out that this 2 years research would have required 30 years of data collection without the efforts of the individuals who sent ticks to Bay Area Lyme Foundation’s Free Tick Testing program. The study’s technique could allow for real-time distribution monitoring of ticks and other relevant species, an important consideration with emerging pathogens, changing land-use patterns, and climate change.

This program continued through 2019 with a Bay Area Lyme Foundation program grant to cover additional Bartonella testing, a disease-causing pathogen carried by ticks.

Bay Area Lyme Foundation’s Free Tick Testing Program was a citizen science success.  Thank you to all who supported this effort by sending in ticks. Stay tuned for additional meaningful ecology results from the Free Tick Testing data collection.

While this program is no longer accepting ticks for testing, there are a number of other tick testing options available.

The full research publications can be found in PLOS ONE and  International Journal of Health Geographics.